What’s up in the cannabis industry

The citations for my talk in Holland SV40 oncogenic propertiesDean (Nucleear Targeting)

PubMed

Sequence requirements for plasmid nuclear import - PubMed

The nuclear envelope is a major barrier for nuclear uptake of plasmids and represents one of the most significant unsolved problems of nonviral gen...

(P53 inhibition)

PubMed

p53 elevation in human cells halt SV40 infection by inhibiting T-ag expression - PubMed

SV40 large T-antigen (T-ag) has been known for decades to inactivate the tumor suppressor p53 by sequestration and additional mechanisms. Our prese...

(Somatic Hypermutability elements)

PubMed

The SV40 virus enhancer functions as a somatic hypermutation-targeting element with potential tumorigenic activity - PubMed

Simian virus 40 (SV40) is a monkey virus with tumorigenic potential in rodents and is associated with several types of human cancers, including lym...

(SV40 plasmids integrate)

PubMed

Durability of transgene expression and vector integration: recombinant SV40-derived gene therapy vectors - PubMed

Many applications of gene delivery require long-term transgene expression. In dividing cells, this result necessitates vector genome persistence, u...

et al (cytosolic DNA and cGAS-STING)

PubMed

The Cytosolic DNA-Sensing cGAS-STING Pathway in Cancer - PubMed

The recognition of DNA as an immune-stimulatory molecule is an evolutionarily conserved mechanism to initiate rapid innate immune responses against...

FluorometrySutton (DNA/RNA hybrids)https://pmc.ncbi.nlm.nih.gov/articles/PMC1218094/Georgiou (Fluorometry under measures DNA 70% after DNaseI)

PubMed

Assay for the quantification of intact/fragmented genomic DNA - PubMed

This study shows that the accuracy of the quantification of genomic DNA by the commonly used Hoechst- and PicoGreen-based assays is drastically aff...

(Fluorometry Cross talk with RNA dyes much worse than DNA dyes)https://www.sciencedirect.com/science/article/abs/pii/S0003269798929141?via%3DihubAnandamide (Fluorometry deep dive)

Fluorometry Deep Dive

The role for DNaseI and RNaseA

Peer Reviewed papers that have Vax DNA detectable in their NCBI SRALee (Nun study with vax DNA and cGAS-STING)https://www.cell.com/action/showPdf?pii=S2211-1247%2822%2900432-6Knabl (Stills Disease and cGAS-STING)

PubMed

Immune transcriptome and antibody response in adult-onset Still's disease with mild flare following administration of mRNA vaccine BNT162b2 - PubMed

Immune transcriptome and antibody response in adult-onset Still's disease with mild flare following administration of mRNA vaccine BNT162b2

(vax DNA in the vaccinated blood samples)

PubMed

A systems immunology study comparing innate and adaptive immune responses in adults to COVID-19 mRNA and adenovirus vectored vaccines - PubMed

Identifying the molecular mechanisms that promote optimal immune responses to coronavirus disease 2019 (COVID-19) vaccination is critical for futur...

DNA in the vaccinated blood samples)

PubMed

Systems biology analysis reveals distinct molecular signatures associated with immune responsiveness to the BNT162b COVID-19 vaccine - PubMed

German Center for Infection Research, German Center for Lung Research, German Research Foundation, Excellence Strategy EXC 2155 "RESIST" and Europe...

(evidence of Ryan et al & Odak)

OSF

Spike DNA and RNA persistenceKrauson (30 days in heart tissue)

PubMed

Duration of SARS-CoV-2 mRNA vaccine persistence and factors associated with cardiac involvement in recently vaccinated patients - PubMed

At the start of the COVID-19 pandemic, the BNT162b2 (BioNTech-Pfizer) and mRNA-1273 (Moderna) mRNA vaccines were expediently designed and mass prod...

(117 days in Cerebral arteries)

PubMed

Expression of SARS-CoV-2 spike protein in cerebral Arteries: Implications for hemorrhagic stroke Post-mRNA vaccination - PubMed

Although the possibility of spike protein expression due to asymptomatic SARS-CoV-2 infection cannot be entirely excluded, this study demonstrated ...

(60 days in Lymph nodes)

PubMed

Immune imprinting, breadth of variant recognition, and germinal center response in human SARS-CoV-2 infection and vaccination - PubMed

During the SARS-CoV-2 pandemic, novel and traditional vaccine strategies have been deployed globally. We investigated whether antibodies stimulated...

(28 days in plasma)https://onlinelibrary.wiley.com/doi/10.1111/apm.13294Hanna (5 days in Breast Milk)https://jamanetwork.com/journals/jamapediatrics/fullarticle/2796427https://www.thelancet.com/journals/ebiom/article/PIIS2352-3964(23)00366-3/fulltextGonzalez (Placenta 2-10 days)

PubMed

Minimal mRNA uptake and inflammatory response to COVID-19 mRNA vaccine exposure in human placental explants - PubMed

Despite universal recommendations for COVID-19 mRNA vaccination in pregnancy, uptake has been lower than desired. There have been limited studies o...

Spike Protein PersistencePatterson (245 days of vax Spike protein)

PubMed

Detection of S1 spike protein in CD16+ monocytes up to 245 days in SARS-CoV-2-negative post-COVID-19 vaccine syndrome (PCVS) individuals - PubMed

Despite over 13 billion SARS-CoV-2 vaccine doses administered globally, persistent post-vaccination symptoms, termed post-COVID-19 vaccine syndrome...

(~700 days of vax Spike Protein)

medRxiv

Immunological and Antigenic Signatures Associated with Chronic Illnesses after COVID-19 Vaccination

COVID-19 vaccines have prevented millions of COVID-19 deaths. Yet, a small fraction of the population reports a chronic debilitating condition afte...

Methylated DNAWang (Plasmid DNA activates cGAS-STING more than PCR)https://pmc.ncbi.nlm.nih.gov/articles/PMC11638879Luecke (Methyl DNA -cGAS-STING)https://www.embopress.org/doi/full/10.15252/embr.201744017Balzarolo ( methyl A activates cGAS-STING more than PCR)https://pubmed.ncbi.nlm.nih.gov/33715389/Carnes (Plasmids for transfection should be unmethylated)

PubMed

Plasmid DNA fermentation strain and process-specific effects on vector yield, quality, and transgene expression - PubMed

Industrial plasmid DNA manufacturing processes are needed to meet the quality, economy, and scale requirements projected for future commercial prod...

DNA contam papersSpeicher (https://pubmed.ncbi.nlm.nih.gov/40913499/)Kammerer https://publichealthpolicyjournal.com/biontech-rna-based-covid-19-injections-contain-large-amounts-of-residual-dna-including-an-sv40-promoter-enhancer-sequence/Konighttps://www.mdpi.com/2409-9279/7/3/41Raoulthttps://hal.science/hal-04778576v1/documentFlemminghttps://www.heraldopenaccess.us/openaccess/quantitative-analysis-of-nucleic-acid-content-in-spikevax-moderna-and-bnt162b2-pfizer-covid-19-vaccine-lotsWanghttps://jhss.scholasticahq.com/article/127890-a-rapid-detection-method-of-replication-competent-plasmid-dna-from-covid-19-mrna-vaccines-for-quality-control?utm_source=chatgpt.comMcKernanhttps://osf.io/preprints/osf/bcsa6Mckernanhttps://osf.io/preprints/osf/b9t7m_v1McKernanhttps://zenodo.org/records/17281691McKernan (Why Achs et al is missing DNA) Leehttps://anandamide.substack.com/p/independent-sanger-sequencing-verificationKaiser (Find it at or under the limit but uses flawed methods that filter the DNA. Funded indirectly by BioNtech)

PubMed

Quantification of objective concentrations of DNA impurities in mRNA vaccines - PubMed

Our results reproducibly confirm DNA:RNA mass ratios of 1:1000 which is consistent with the specifications of the approved mRNA vaccine product. We...

Papers on plasmid DNA integrationsDuncan (1,000s of integrations in 99% of patients with lentiviral plasmids)https://www.nejm.org/doi/full/10.1056/NEJMoa2405541Lim (spontaneous integrations with plasmids and LNPs)https://pubmed.ncbi.nlm.nih.gov/37100816/Sheng-Fowler (fg-ag DNA limits for small DNA)https://pubmed.ncbi.nlm.nih.gov/19285882/Moderna US patent 10,898,574 (Insertional mutagenesis with DNA)

US10898574B2 - Delivery and formulation of engineered nucleic acids - Google Patents

patent 10,702,600 (Insertional mutagenesis with DNA)

US10702600B1 - Betacoronavirus mRNA vaccine - Google Patents

patent 10,077,439 (qPCR undermeasures it)

US10077439B2 - Removal of DNA fragments in mRNA production process - Google Patents

The present invention describes methods of removing DNA from an RNA transcript during the mRNA production process. The method embodies procedures f...

EMA documents

Covid Truths

EMA Leaked Papers - Covid Truths

There is an analysis from the BMJ after the EMA leaked papers, which consist of pictures, PDFs and docs. I’ll also post it here where you can...

Chicken Dinner- 10X coverage of a salmonella genome in 10minutes. Nanopores. GPU acceleration for real time basecalling and genome alignment. This took a a few hours to turn a colony into a Nanopore motor protein adapter library but the sequencing pushed out a 70X genome in 40 minutes. Next up, Dozen at a time.

Trey is in surgery tonight. Obstructed small intestine Fur ball

The Pink Kush genome now etched into Bitcoin. Learn more about this at

Kannapedia

Pink Kush | Kannapedia

I want to thank everyone who has zapped me since I have been here. Im currently burning some sats to BTC etch Critical links to papers I place on NOSTR. I am using very lightweight links and hashes and not 'spamming' the chain. I think I found a way to use my supporters sats on this platform to make that happen. Burned through about 5000 sats already at 10sats/b with slipstream etching multiple papers related to Pfizers DNA contamination. The more folks zap... the more I'll etch. I have about 200 substacks on the topic. Each I can turn into a PNG file , post it here and etch the link/hash there.

Hyperstimulatory N⁶-methyladenine (m6A) in residual SV40 plasmid DNA in mRNA vaccines. Being hashed and stashed on BTC through slipstream.

Final version submitted.

Zenodo

Critical Review of Achs et al. (2025) on Residual DNA in mRNA Vaccine Preparations

In a preprint, Achs et al. (2025) present an analysis from the Biomedical Research Center of the Slovak Academy of Sciences (BMC SAS) of residual ...

Latest Version. Added Achs to the References and corrected a few typos.

A review of Achs et al.

A frame work for a new "Peer to Peer" peer review system using @primal_app and Bitcoin.

LOL- Warren Buffet is here to help you invest in Crypto.

Our DNA contamination paper etched into Nostr. Going to try to put a link to this Nostr thread in the new OP_RETURN. Shasum -a 256 a486c8542c0938222520a20d6db33121a8eebe329716ceb5221848f4bb63d2cf

Nanopore Sequencing

Revealing the bacterial methylation signals in Pfizers DNA contaminants.

The Cut Gloucester Mass Dead to the Core

Pharmocracy

Lonni parroting Chesire who was bagged lying about this PubSmear complaint by @VaccineMole. Even though Kevin Patrick admitted to lying, Lonni hasn’t removed the defamation.

It’s such a harmless amount of DNA in the shots that they cancel anyone who talks about it.